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ADE - ADVANCED MICROSCOPY TECHNIQUES FOR BIOMEDICAL APPLICATIONS

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Academic year 2023/2024

Course ID
SCB0363
Teacher
Saverio Francesco Retta (Lecturer)
Year
3rd year
Teaching period
Second semester
Type
Elective
Credits/Recognition
1
Course disciplinary sector (SSD)
BIO/13 - experimental biology
Delivery
Lecture
Language
English
Attendance
Mandatory
Type of examination
Oral
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Sommario del corso

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Course objectives

Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.

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Results of learning outcomes

Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.

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Program

  • Fundamental principles of microscopic observation
  • Techniques for the preparation of cells and tissues for microscopic observation
  • Phase contrast microscopy for living cells
  • Interferential Contrast Microscopy (DIC)
  • Fluorescence microscopy: principles and applications
  • Confocal microscopy
  • Multifluorescence imaging
  • Time-lapse microscopy
  • Fluorescent chimeric proteins: experimental strategies to monitor the dynamics of a protein and its relationships with subcellular structures in living cells
  • The "F" techniques and their applications: "Fluorescence Resonance Energy Transfer" (FRET), "Fluorescence Recovery After Photobleaching" (FRAP), "Fluorescence Loss In Photobleaching" (FLIP), "Fluorescence Lifetime Imaging Microscopy" (FLIM), etc.
  • Two-photon microscopy
  • Correlative microscopy
  • Super-resolution microscopy
  • Clinical applications of fluorescence microscopy
  • Principles and applications of electron microscopy
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Course delivery

  • Face-to-face lectures/seminars
  • Streaming (Webex/Zoom) lectures/seminars held by national and international experts in the field of advanced microscopy techniques 
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Learning assessment methods

As an Elective Didactic Activity (ADE) chosen by the student, this ADE aims to personalize the student's educational path on the basis of their inclinations and interests, deepening specific knowledge and training aspects by discussion of topics that are not included in the "core curriculum" of the Integrated Courses.

The acquisition of the credits assigned to ADE takes place only upon reaching 100% of attendance.

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Support activities

Seminars of specialists in fluorescence and electron microscopy techniques

Suggested readings and bibliography

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Didactic material in the form of reviews and research articles will be provided by the teacher.



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Notes

  • Maximum number of students: 40
  • Schedule: from June 3rd to June 5th, 2024, from 9:00 to 13:00

 

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Class scheduleV

Lessons: from 03/06/2024 to 05/06/2024

Notes: from h. 9:00 to 13:00

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Last update: 15/01/2024 13:30
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