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ADE - ADVANCED MICROSCOPY TECHNIQUES FOR BIOMEDICAL APPLICATIONS

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Ade - advanced microscopy techniques for biomedica applications

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Academic year 2020/2021

Course ID
SCB0363
Teacher
Prof. Saverio Francesco Retta (Lecturer)
Year
1st year 2nd year 3rd year
Teaching period
Second semester
Type
Elective
Credits/Recognition
1
Course disciplinary sector (SSD)
BIO/13 - biologia applicata
Delivery
Lecture
Language
English
Attendance
Mandatory
Type of examination
Oral
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Sommario del corso

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Course objectives

Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.

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Results of learning outcomes

-

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Course delivery

Webex lectures/seminars

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Learning assessment methods

-

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Support activities

Seminars of specialists in fluorescence and electron microscopy techniques

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Program

  • Fundamental principles of microscopic observation
  • Techniques for the preparation of cells and tissues for microscopic observation
  • Phase contrast microscopy for living cells
  • Interferential Contrast Microscopy (DIC)
  • Fluorescence microscopy: principles and applications
  • Confocal microscopy
  • Multifluorescence imaging
  • Time-lapse microscopy
  • Fluorescent chimeric proteins: experimental strategies to monitor the dynamics of a protein and its relationships with subcellular structures in living cells
  • The "F" techniques and their applications: "Fluorescence Resonance Energy Transfer" (FRET), "Fluorescence Recovery After Photobleaching" (FRAP), "Fluorescence Loss In Photobleaching" (FLIP), "Fluorescence Lifetime Imaging Microscopy" (FLIM), etc.
  • Two-photon microscopy
  • Correlative microscopy
  • Super-resolution microscopy
  • Clinical applications of fluorescence microscopy
  • Principles and applications of electron microscopy

Suggested readings and bibliography

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To be defined



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Class schedule

DaysTimeClassroom
Tuesday9:00 - 13:00

Lessons: dal 11/05/2021 to 25/05/2021

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Note

Maximum number of students: 40

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Last update: 12/04/2021 09:13
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