- Oggetto:
- Oggetto:
ADE - ADVANCED MICROSCOPY TECHNIQUES FOR BIOMEDICAL APPLICATIONS
- Oggetto:
Ade - advanced microscopy techniques for biomedica applications
- Oggetto:
Academic year 2021/2022
- Course ID
- SCB0363
- Teacher
- Prof. Saverio Francesco Retta (Lecturer)
- Year
- 3rd year 4th year
- Teaching period
- Second semester
- Type
- Elective
- Credits/Recognition
- 1
- Course disciplinary sector (SSD)
- BIO/13 - biologia applicata
- Delivery
- Lecture
- Language
- English
- Attendance
- Mandatory
- Type of examination
- Oral
- Oggetto:
Sommario del corso
- Oggetto:
Course objectives
Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.
- Oggetto:
Results of learning outcomes
-
- Oggetto:
Course delivery
Face-to-face and streaming (Webex/Zoom) lectures/seminars
- Oggetto:
Learning assessment methods
-
- Oggetto:
Support activities
Seminars of specialists in fluorescence and electron microscopy techniques
- Oggetto:
Program
- Fundamental principles of microscopic observation
- Techniques for the preparation of cells and tissues for microscopic observation
- Phase contrast microscopy for living cells
- Interferential Contrast Microscopy (DIC)
- Fluorescence microscopy: principles and applications
- Confocal microscopy
- Multifluorescence imaging
- Time-lapse microscopy
- Fluorescent chimeric proteins: experimental strategies to monitor the dynamics of a protein and its relationships with subcellular structures in living cells
- The "F" techniques and their applications: "Fluorescence Resonance Energy Transfer" (FRET), "Fluorescence Recovery After Photobleaching" (FRAP), "Fluorescence Loss In Photobleaching" (FLIP), "Fluorescence Lifetime Imaging Microscopy" (FLIM), etc.
- Two-photon microscopy
- Correlative microscopy
- Super-resolution microscopy
- Clinical applications of fluorescence microscopy
- Principles and applications of electron microscopy
Suggested readings and bibliography
- Oggetto:
Didactic material and articles provided by the teacher.
- Oggetto:
Class schedule
Days Time Classroom Tuesday 9:00 - 13:00 Lessons: dal 11/05/2022 to 25/05/2022
Notes: 11/05/2022
13/05/2022
25/05/2022
from h 9;00 to h 13;00- Oggetto:
Note
Maximum number of students: 40
- Oggetto: