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ADE - ADVANCED MICROSCOPY TECHNIQUES FOR BIOMEDICAL APPLICATIONS
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Ade - advanced microscopy techniques for biomedica applications
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Academic year 2022/2023
- Course ID
- SCB0363
- Teacher
- Saverio Francesco Retta (Lecturer)
- Year
- 3rd year 4th year
- Teaching period
- Second semester
- Type
- Elective
- Credits/Recognition
- 1
- Course disciplinary sector (SSD)
- BIO/13 - experimental biology
- Delivery
- Lecture
- Language
- English
- Attendance
- Mandatory
- Type of examination
- Oral
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Sommario del corso
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Course objectives
Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.
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Results of learning outcomes
Knowledge of the main techniques and applications of conventional and confocal optical microscopy in the field of basic and applied biomedical research.
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Course delivery
- Face-to-face lectures/seminars
- Streaming (Webex/Zoom) lectures/seminars held by national and international experts in the field of advanced microscopy techniques
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Learning assessment methods
As an Elective Didactic Activity (ADE) chosen by the student, this ADE aims to personalize the student's educational path on the basis of their inclinations and interests, deepening specific knowledge and training aspects by discussion of topics that are not included in the "core curriculum" of the Integrated Courses.
The acquisition of the credits assigned to ADE takes place only upon reaching 100% of attendance.
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Support activities
Seminars of specialists in fluorescence and electron microscopy techniques
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Program
- Fundamental principles of microscopic observation
- Techniques for the preparation of cells and tissues for microscopic observation
- Phase contrast microscopy for living cells
- Interferential Contrast Microscopy (DIC)
- Fluorescence microscopy: principles and applications
- Confocal microscopy
- Multifluorescence imaging
- Time-lapse microscopy
- Fluorescent chimeric proteins: experimental strategies to monitor the dynamics of a protein and its relationships with subcellular structures in living cells
- The "F" techniques and their applications: "Fluorescence Resonance Energy Transfer" (FRET), "Fluorescence Recovery After Photobleaching" (FRAP), "Fluorescence Loss In Photobleaching" (FLIP), "Fluorescence Lifetime Imaging Microscopy" (FLIM), etc.
- Two-photon microscopy
- Correlative microscopy
- Super-resolution microscopy
- Clinical applications of fluorescence microscopy
- Principles and applications of electron microscopy
Suggested readings and bibliography
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Didactic material in the form of reviews and research articles will be provided by the teacher.
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Class schedule
Days Time Classroom Tuesday 9:00 - 13:00 Lessons: from 18/05/2023 to 25/05/2023
Notes: 18/05/2023
19/05/2023
25/05/2023
from h 9;00 to h 13;00- Oggetto:
Note
Maximum number of students: 40
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